Topic outline
Proteins and Enzymes
Module 1
LECTURES (3)
- Introduction. A subject and tasks of biochemistry. Physical and chemical bases of biochemical processes. Structure of proteins. Structural organization and properties of proteins Interaction of proteins with ligands as a basis of functioning of proteins. The active center. Interrelation of structure and function proteins.
- LECTURE 2 : -
Enzymes. A biological role. The mechanism and features catalytic efficiency of enzymes. Cofactors and соenzymes.
LECTURE 3 : .
Kinetics of enzymatic catalysis. Principles of determination enzymatic activity. Medical enzymology (enzymes in biotechnology and medicine).
CLASSES (7)
Unit 1.(11.01 - 16.01) Methods of biochemical research. Structures of amino acids and peptides
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Unit 2.(18.01 - 23.01) STRUCTURE, PHYSICO-CHEMICAL PROPERTIES AND FUNCTIONS OF PROTEIN
TEST IN WRITTEN FORM:
1)Name structural formulae of 20 amino acids
2) Write the formula of the peptide---------------------
Unit 3.(25.01 - 30.01) CLASSIFICATION OF PROTEINS. PROTEIN-LIGAND INTERACTION. PROTEIN STRUCTURE-FUNCTION RELATIONSHIPS
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Unit 4.(22.09 - 28.09) PROPERTIES OF ENZYMES, COENZYMES. CATALYTIC EFFICIENCY OF ENZYMES
Test in written form: the structural formulae of NAD+, NADP+, FAD, TPP, PLP, Biotin, tetrahydrofolic acid, CoASH.
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Unit 5.(29.09 - 5.10) ENZYME KINETICS. FACTORS AFFECTING REACTION VELOCITY. ENZYMES IN MEDICINE
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Unit 6.(6.10 - 12.10) Regulation of enzyme activity. Enzyme activity inhibition
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Unit 7. (13.10 - 19.10) Colloquium: Proteins. Enzymes.
Bioenergetics
Module 2
LECTURES (3)
- LECTURE 4: Biological oxidation. Mitochondrial electron transport chain. ATPsyntase. Regulation of oxidative phosphorylation. Disorders of mitochondrial oxidative phosphorylation.
- LECTURE 5: .- gr.- Carbohydrates: structure, biological role, classification. Digestion and absorption of food carbohydrates. Synthesis and catabolism of glycogen. Regulation of glucose storage and mobilization.
LECTURE 6: -
Glycolysis. Gluconeogenesis. Fermentation. Pentose phosphate pathway. Disorders of carbohydrate metabolism.
CLASSES (6)
Unit 8. Energy metabolism and ATP formation. Mitochondrial electron transport chain
Read!!! Main Textbook : Satyanarayana from page 221 (Chapter 11).
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Unit 9. Common catabolic pathway. Tricarboxylic acid cycle
Read!!! Main Textbook : Satyanarayana Chapter 12 and Chapter 13.
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Marks' Basic Medical Biochemistry
Unit 10. Carbohydrate metabolism. Digestion of dietary carbohydrates. Glycogen metabolism. Regulation of glycogen metabolism
Read!!! Main Textbook : Satyanarayana Chapter 2 and Chapter 13.
- Test in written form:
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Marks' Basic Medical Biochemistry
Unit 11. Catabolism of glucose
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Unit 12. Gluconeogenesis. Regulation of glycolysis and gluconeogenesis. Pentose phosphate pathway
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Unit 13. Colloquium: Bioenergetics. Carbohydrates
- Read Main Textbook : Satyananarayana from page 221.
Lipids. Biological membranes
Module 3
LECTURES (3)
LECTURE 7:
Lipids: structure, biorole, classification. Digestion of lipids and absorption of products of digestion in the gastrointestinal tract. Transport of lipids, lipoproteins. Storage and mobilization of fats in adipose tissue. Regulation.
LECTURE 8:
β-oxidation of fatty acids. Oxidative degradation of glycerol. Biosynthesis of fatty acids. Regulation. Synthesis of triacylglycerols and phospholipids.
LECTURE 9:
Synthesis and utilization of ketone bodies in organism. Metabolic acidosis. Biorole of cholesterol. Synthesis of cholesterol. Bile acids. Eicosanoids. Disturbances of lipids metabolism in organism.
CLASSES (5)
Unit 14.
DIGESTION AND ABSORPTION OF LIPIDS. LIPOPROTEINS
Test in written form!!!
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Unit 15. Lipid metabolism I
- Additions
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Unit 16. Lipid metabolism II.
By Peter Forster - Own work, CC BY-SA 4.0, https://commons.wikimedia.org/w/index.php?curid=45808291
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Unit 17. Colloquium: Lipids
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Unit 18.Structure and functions of membranes. Role of membranes in signal transduction
Module 4. (4 semester - Spring term)
Aminoacids metabolism
LECTURES (3)
LECTURE 10:
Common pathway of amino acids. Deamination. Kinds of direct deamination. Oxidases of amino acids and glutamate dehydrogenase. Synthesis and degradation of glutamine. Transamination of amino acids Utilization of ammonia in urea cycle and removing of urea.
LECTURE 11:
Decarboxylation of amino acids. Biorole of biogenic amines. Polyamines. Degradation of biogenic amines. Metabolism of phenylalanine and tyrosine. Hereditary defects of phenylalanine catabolism (alcaptonuria and phenylketoneuria).