Topic outline
Lectures
Link for Lectures
Time
LinkLECTURE 1.
Introduction. A subject and tasks of biochemistry. Physical and chemical bases of biochemical processes. Structure of proteins. Structural organization and properties of proteins Interaction of proteins with ligands as a basis of functioning of proteins. The active center. Interrelation of proteins structure and function.Supplement
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LECTURE 2.
Enzymes. A biological role. The mechanism and features catalytic efficiency of enzymes. Cofactors and соenzymes.Supplement
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LECTURE 3.
Kinetics of enzymatic catalysis. Principles of determination enzymatic activity. Medical enzymology (enzymes in biotechnology and medicine).---------------------
LECTURE 4.
Biological oxidation. Mitochondrial electron transport chain. ATP-syntase. Regulation of oxidative phosphorylation. Disorders of mitochondrial oxidative phosphorylation.- ---------------------
LECTURE 5.
Carbohydrates: structure, biological role, classification. Digestion and absorption of food carbohydrates. Synthesis and catabolism of glycogen. Regulation of glucose storage and mobilization.- ---------------------
- LECTURE 6.
Glycolysis. Gluconeogenesis. Fermentation. Pentose phosphate pathway. Disorders of carbohydrate metabolism. - ---------------------
- LECTURE 7.
Lipids: structure, biorole, classification. Digestion of lipids and absorption of products of digestion in the gastrointestinal tract. Transport of lipids, lipoproteins. Storage and mobilization of fats in adipose tissue. Regulation. - ---------------------
- LECTURE 8.
Beta-oxidation of fatty acids. Oxidative degradation of glycerol. Biosynthesis of fatty acids. Regulation. Synthesis of triacylglycerols and phospholipids. - ---------------------
- LECTURE 9.
Synthesis and utilization of ketone bodies in organism. Metabolic acidosis. Biorole of cholesterol. Synthesis of cholesterol. Bile acids. Eicosanoids. Disturbances of lipids metabolism in organism. - ---------------------
Proteins and Enzymes
Module 1 (5 Classes)
- Unit 1. Introduction to biochemistry. Physico-chemical properties of proteins. Levels of organization of proteins. .
- Manuals
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- Unit 2. Structure, properties and functions of proteins. The protein–ligand interaction. Quantitative assay of proteins in solution .
TEST IN WRITTEN FORM:
1)Name structural formulae of 20 amino acids
2) Write the formula of the peptide- Manuals
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- Unit 3. Enzymes. Biological role. Mechanism and features catalytic efficiency of enzymes. Cofactors and coenzymes .
- Test in written form:
The structural formulae of : NAD+, NADP, FAD, TPP, PLP, Biotin, tetrahydrofolic acid, CoASH. - Manuals
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- Unit 4. Kinetics of enzyme-catalyzed reactions. Principles of assay activity of enzymes.Medical enzymology (enzymopathology, enzymodiagnostics and enzymotherapy).
- Readings: Main TextBook (Satyanarayana)-
Q1: pages 88-91 - ---------------------
- Unit 5. Regulation of enzyme activity as molecular basis of regulation of metabolism by external signals. Inhibition of enzyme activity.
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Bioenergetics
Module 2 (6 Classes)
- Unit 6. Bioenergetics of the cell. Mitochondrial electron transport chain.
Read!!! Main Textbook : Satyanarayana from page 221 (Chapter 11).
- Supplement
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- Unit 7. Common catabolic pathway. Tricarboxylic acid cycle.
Read!!! Main Textbook : Satyanarayana Chapter 12 and Chapter 13.
- Supplement
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- Unit 8. Carbohydrate metabolism. Digestion of dietary carbohydrates. Glycogen metabolism. Regulation of glycogen metabolism.
Read!!! Main Textbook : Satyanarayana Chapter 2 and Chapter 13.
- Test in written form:
- Supplement
- Marks' Basic Medical Biochemistry
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- Unit 9. Catabolism of glucose.
Read!!! Main Textbook ( Satyanarayana): pages 244-251 (Fig.13.2); pages 234-235 (Shuttles -Fig.11.12 and 11.13)
- Supplement
- Marks' Basic Medical Biochemistry
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- Unit 10. Gluconeogenesis. Regulation of glycolysis and gluconeogenesis. Pentose phosphate pathway.
- Supplement
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- Unit 11. Colloquium: Bioenergetics. Carbohydrates.
Read Main Textbook : Satyananarayana from page 221
Lipids.
Module 3 (5 Classes)
- Unit 12. Digestion and Absorbption of Lipids. Lipiproteins.
Test in written form!!!
- Supplement
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- Unit 13. Lipid metabolism I.
- Supplement
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- Unit 14. Lipid metabolism II.
- Supplement
By Peter Forster - Own work, CC BY-SA 4.0, https://commons.wikimedia.org/w/index.php?curid=45808291
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- Unit 15. Colloquium: Lipids.
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